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Local and Systemic Induction of β‐1,3‐Glucanase and Chitinase in Coffee Leaves Protected Against Hemileia vastatrix by Bacillus thuringiensis
Author(s) -
Guzzo S. D.,
Martins E. M. F.
Publication year - 1996
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1996.tb00322.x
Subject(s) - glucanase , chitinase , biology , inducer , bacillus thuringiensis , botany , horticulture , enzyme , biochemistry , bacteria , genetics , gene
β‐1,3‐glucanase and chitinase activities were induced locally and systemically 4–25 and 11–25 days, respectively, after spraying the surface of the third pair of coffee leaves from the apex of 8‐month‐old plants with a 50 mg/ml aqueous suspension of Bacillus thuringiensis in a commercial formulation (Thuricide HP‐Sandoz). The treatment also induced local and systemic resistance against Hemileia vastatrix after the application of the inducer. Within 14–18 days of application of the Thuricide inducer, the β‐1,3‐glucanase activity in the locally and systemically‐protected unchallenged leaves reached maximum levels of 226% and 279% higher levels respectively, than in control plants. The chitinase activity reached maximum levels of 224% and 181% respectively, within 18–21 days after treatment with the inducer. Two β‐1,3‐glucanase bands were detected by native PAGE electrophoresis in extracts from locally‐and systemicallyprotected unchallenged coffee leaves.