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Biological Characterization of an Italian Isolate of Barley Yellow Dwarf Luteovirus from Barley
Author(s) -
Guglielmone L.,
Caciagli P.
Publication year - 1996
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1996.tb00310.x
Subject(s) - barley yellow dwarf , luteovirus , biology , rhopalosiphum maidis , antiserum , polyclonal antibodies , myzus persicae , rhopalosiphum padi , dwarfing , inoculation , hordeum vulgare , botany , horticulture , poaceae , plant virus , virus , virology , homoptera , antibody , aphididae , pest analysis , aphid , rootstock , immunology
An isolate of BYDV (BYDV‐OC), from barley in Northwest Italy with typical symptoms of yellowing and dwarfing, was transmitted by Rhopalosiphum padi, Sitobion fragariae. S. avenae, Metopolophium festucae, R. maidis and M. dirhodum , but not by Myzus persicae or Schizaphis graminum . It reacted in DAS‐ELISA with monoclonal and polyclonal antisera to PAV, but not with antibodies to MAV, RPV and RMV. A polyclonal antiserum prepared to BYDV‐OC did not react with MAV‐like, RPV‐like, or RMV‐like isolates of BYDV in ELISA or in Western blots. The concentration of BYDVOC in Avena byzantina plants decreased from weeks 1 to 10 after inoculation, but the total virus content per plant increased up to weeks 7 to 8, following the increase of plant weight.