Premium
Routing of the G Protein During Maturation of Festuca Leaf Streak Rhabdovirus in its Plant Host
Author(s) -
Lundsgaard T.
Publication year - 1995
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1995.tb04558.x
Subject(s) - biology , golgi apparatus , endoplasmic reticulum , phloem , parenchyma , secretory pathway , immunogold labelling , microbiology and biotechnology , vesicular stomatitis virus , festuca , secretory protein , virus , botany , virology , biochemistry , ultrastructure , secretion , poaceae
By immunogold labelling the location of Festuca leaf streak virus glycoprotein (FLSV‐G) was investigated in developing phloem and mature leaf parenchyma of Festuca gigantea infected with Festuca leaf streak virus (FLSV: Rhabdotiridae ). In developing phloem cells, FLSV‐G was detected in endoplasmic reticulum (ER). at perinuclear membranes, and in assembled virions, but neither in Golgi stacks and Golgi vesicles nor at the plasma membrane of infected cells. These results indicate that FLSV‐G stays in the ER after transmembrane synthesis, and is not routed through the secretory pathway in F. gigantea. The membranous inclusions, present in infected mature leaf parenchyma cells were found to contain FLSV‐G. It is suggested that the, virus‐induced membranous inclusions have developed from FLSV‐G‐containing ER. The residence of FLSV‐G in ER (present study) is in contrast to results with vesicular stomatitis virus (VSV; vertebrate rhabdovirus). Here the G protein is known to be routed to the plasma membrane through the secretory pathway.