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Weitere Versuchsergebnisse, die eine Einstufung des Ullucus mild mottle virus als eigenes Tobamovirus rechtfertigen
Author(s) -
Offei S. K.,
Ibidapo O.,
Brunt A. A.,
Coutts R. H. A.
Publication year - 1995
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1995.tb00660.x
Subject(s) - tobamovirus , biology , mottle , tobacco mosaic virus , virology , mosaic virus , rna , plant virus , brome mosaic virus , microbiology and biotechnology , virus , genetics , rna dependent rna polymerase , gene
The affinities of Ullucus mild mottle virus (UMMV). purified by a modified procedure. were examined by immunoblotting and probing with antisera to five distinct tobamoviruses. RNA. isolated from purified virus, was used for in vitro protein translation in a wheat germ system and the products examined by denaturing polyacrylamide gel electrophoresis. The results of these investigations, together with a study of the double‐stranded RNAs associated with infection. confirm that UMMV is a distinct, tobamovirus which has close affinities with tobacco mosaic, tomato mosaic and cucumber green mottle tobamoviruses and more distant relationships with ribgrass mosaic and odontoglossum ringspot tobamoviruses.