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Genetic Diversity in W‐ and R‐type Populations of Pseudocercosporella herpotrichoides Based on DNA Restriction Fragment Length Polymorphisms
Author(s) -
Poupard P.,
Frei U.,
Cavelier* N.,
Lind V.
Publication year - 1995
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1995.tb00239.x
Subject(s) - biology , restriction fragment length polymorphism , genetics , genetic diversity , genotype , dna , restriction fragment , polymorphism (computer science) , gene , population , demography , sociology
Thirty‐seven isolates of Pseudocercosporella herpotrichoides belonging to W‐type and 31 isolates belonging to R‐type were analysed for DNA restriction fragment length polymorphisms (RFLPs). They represent diverse geographic origins and different phenotypes related to sensitivity to fungicides. Total DNA digested with Eco RI was hybridized with 22 random DNA probes from a P. herpotrichoides Eco RI‐restricted DNA library. Four probes showing polymorphisms among isolates within W‐type and R‐type and generating a total of 44 RFLPs were retained for cluster analysis. Two main groups were distinguished corresponding to W‐ and R‐types. The genetic diversity among isolates was greater for the W‐type than for the R‐type isolate, four and three distinct Eco RI‐restricted mitochondrial DNA patterns were identified in W‐ and R‐type isolates, respectively. The variability of profiles within each pathotype confirmed a higher degree of polymorphism in the W‐type.