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Characterization of the N Gene of a Groundnut Ringspot Virus (GRSV) Isolate from Argentina
Author(s) -
Dewey R. A.,
Semorile L.,
Grau O.,
Avila A. C.,
Kitajima E. W.
Publication year - 1995
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1995.tb00228.x
Subject(s) - biology , homology (biology) , gene , virology , nucleic acid sequence , virus , sequence homology , genetics , sequence analysis , peptide sequence
This paper describes the characterization of a Tospotirus isolate (M316) from Mendoza, Argentina. The N gene was cloned and sequenced. The sequence obtained was compared with the N gene sequences of other tospoviruses. The percentages of the nucleotide homology and amino acid similarity showed that this isolate may be considered a member of the species groundnut ringspot virus (GRSV). The conclusion drawn from the molecular studies was confirmed by DAS‐ELISA and western blotting. This is thefirst report of GRSV in Argentina, previously described for a South African (SA‐05) and Brazilian (BR‐08) isolates.