Premium
Variability of Xanthomonas Campestris pv. vasculorum From Sugarcane and Other Gramineae in Reunion Island. Characterization of a Different Xanthomonad
Author(s) -
Péros J. P.,
Girard J. C.,
Lombard H.,
Janse J. D.,
Berthier Y.
Publication year - 1994
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1994.tb04528.x
Subject(s) - biology , xanthomonas campestris , pathovar , restriction fragment length polymorphism , poaceae , xanthomonas , botany , cultivar , microbiology and biotechnology , pseudomonadaceae , genotype , genetics , bacteria , gene , pseudomonas
Stains presumed to be of Xanthomonas campestris pv. vasculorum (Cobb.) Dye, obtained from sugarcane and other gramineae in Réunion Island, were compared in terms of cultural aspects, pathogenic and physiological reactions, fatty acid profiles and restriction fragment length polymorphism (RFLP) of genomic DNA. The strains could be divided into two separate groups (G1 and G2). The G1 strains were identical to strains described as X. campestris pv. vasculorum ; they showedan important variability in their cultural characteristics and in their aggressiveness. The G2 strains did not induce the usual symptoms of gumming disease on sugarcane cultivars infected under natural conditions or inoculated in the greenhouse. The G2 strains grew faster on agar medium, their colonies were more pigmented and less fluidal and had a different morphology on agar slant. Unlike the G1 strains, G2 strains hydrolyzed starch weakly and casein strongly; they utilized L‐fucose and, to a lesser extent, melibioze. The fatty acid and genomic DNA profiles differed between the groups. Differences seemed large enough to support separation of G1 and G2 strains into distinct taxonomic entities, namely G1 as Xanthomonas campestris pv. vasculorum and G2 as a different pathovar of X. campestris. The taxonomic position of G2 strains is discussed.