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Cross‐Reactive Antigens Between Pea and Some Fungal Plant Pathogens
Author(s) -
Scala F.,
Cristinzio G.,
Coppola L.,
Sorbo G.
Publication year - 1994
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1994.tb04518.x
Subject(s) - biology , phytophthora capsici , sclerotinia sclerotiorum , rhizoctonia solani , fusarium solani , fusarium oxysporum , verticillium dahliae , microbiology and biotechnology , sclerotium , fungi imperfecti , pathogen , botany , phytophthora
Antiserum to pea was used to analyse cross‐reactive antigens (CRA) between pea and some fungal plant pathogens with different levels of specificity towards this host by using both double diffusion and immunoblotting techniques. Non pathogens of pea were also included in the study. Nectria haematococca MPVI, the three formae speciales dianthi, lycopersici and pisi of Fusarium oxysporum and Ascochyta pisi produced strong reactions with both techniques. In N. haematococca MPI, F. solani f. sp. phaseoli, V. dahliae and Phoma medicaginis var. pinodella instead, reactions were not detected by double diffusion but only the more sensitive immunoblotting technique. No CRA were observedin, the non‐specific pathogens Rhizoctonia solani, Sclerotium rolfsii and Sclerotinia sclerotiorum , as well as in the non‐pathogen Phytophthora capsici. The immunoblotting patterns of the most reactive fungi showed common bands with molecular weights of 84, 75 and 62 kDa. Some bands were present only in the specific pathogens N. haematococca MPVI and F. oxysporum f.sp. pisi. The possible involvement in host‐parasite interactions of cross‐reactive antigens which are, present in the analyzed fungi is discussed.