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Changes in Peroxidase Activity in Bean Suspension Cultures after B. cinerea and Elicitor Treatment
Author(s) -
Malolepsza U.,
Urbanek H.
Publication year - 1994
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1994.tb01475.x
Subject(s) - elicitor , botrytis cinerea , peroxidase , biology , phaseolus , isoelectric point , guaiacol , isoelectric focusing , mycelium , isozyme , biochemistry , intracellular , isoflavonoid , colletotrichum lindemuthianum , microbiology and biotechnology , enzyme , botany , flavonoid , antioxidant
Treatment of cell suspension cultures of bean ( Phaseolus vulgaris ) c.v. ‘Gold Saxa’) with Botrytis cinerea resulted in the increase in extra‐ and intracellular peroxidase activity. A similar effect was obtained by treatment of cell suspension cultures with an elicitor active extract from fungal mycelium. It was shown by means of electrofocusing that the infection‐ or elicitor‐related increase in total intracellular peroxidase activity was associated with the increase in the activity of a specific isozyme with an isoelectric point of 4.8. This anionic peroxidase did not differ in substrate specificity to guaiacol, syringaldazine and chlorogenic acid.