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Reactivity of Two Monoclonal Antibodies to the Cylindrical Inclusion Protein of Potato Virus Y
Author(s) -
Boudazin G.,
Vergnet C.,
Gélie B.,
Meyer M.,
Grosclaude J.,
Maury Y.
Publication year - 1994
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1994.tb01460.x
Subject(s) - monoclonal antibody , potato virus y , immunogold labelling , biology , virology , epitope , potyvirus , virus , capsid , antibody , microbiology and biotechnology , plant virus , immunology
Monoclonal antibodies (mAbs) were produced to the cylindrical inclusion (CI) protein of a necrotic strain of potato virus Y (PVY‐N) and their reactivity tested with nine PVY isolates and 12 other potyviruses. mAb 95 showed a reactivity range larger than PVY, unlike mAb 88 which only recognised the PVY isolates. Moreover, immunogold labelling of the Cls of PVY‐N, either in situ or in cell extracts, was only obtained with mAb 88, thus showing that the corresponding virus‐specific epitope is located at the surface of the Cl polymer.