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The Level of Abutilon Mosaic Geminivirus in Leaf Discs and Wound Callus
Author(s) -
Song J.Y.,
Jeske H.
Publication year - 1994
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1994.tb01444.x
Subject(s) - callus , abutilon , biology , botany , dna , tissue culture , virus , virology , genetics , in vitro , weed
The level of Abutilon mosaic geminivirus in leaf discs, wound callus and suspension culture was monitored by hybridization. Over 25 days the amount of AbMV DNA decreased in the leaf discs kept under various experimental conditions including different temperatures, light regimes and hormone concentrations. In primary callus or in suspension culture no viral DNA was detectable. When leaf discs were kept in liquid medium instead of on agar plates a shift from viral single‐stranded DNA to replicative double‐stranded DNA was observed when phytohormones were supplied. It is discussed here why under these conditions, rapidly dividing tissues seem to be virus‐free.