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Detection of the 3a Protein of Cucumber Mosaic Virus in a Cell Wall Fraction from Infected Nicotiana clevelandii Plants
Author(s) -
Burman A. J.,
Osman T. A. M.,
Buck K. W.
Publication year - 1994
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1994.tb00028.x
Subject(s) - antiserum , biology , inoculation , capsid , virus , cucumber mosaic virus , virology , nicotiana , plant virus , microbiology and biotechnology , antibody , horticulture , solanaceae , immunology , biochemistry , gene
The 3a protein of cucumber mosaic virus was expressed in Escherichia coli and, after purification, used to produce an antiserum. The 3a protein was detected in a cell wall fraction obtained from infected Nicotiana clevelandii leaf tissue by immunoblotting using the 3a antiserum. The 3a protein reached a maximum level 4 days after inoculation and remained at this level for a further 8 days before slowly declining. In contrast, the virus capsid protein, detected in an 80 000 g pellet by immunoblotting using a virus particle antiserum, reached a maximum 5 days after inoculation and remained at this level for at least a further 16 days.