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Serological Detection of Members of the Potyviridae with Polyclonal Antisera
Author(s) -
Richter J.,
Proll E.,
Rabenstein F.,
Stanarius A.
Publication year - 1994
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1994.tb00002.x
Subject(s) - potyvirus , antiserum , potyviridae , biology , virology , turnip mosaic virus , polyclonal antibodies , immunogen , cross reactivity , immunoelectron microscopy , serology , plant virus , virus , cross reactions , antibody , monoclonal antibody , immunology
The cross‐reactivity of two polyclonal antisera with various members of the Potyviridae was determined using four different serological methods. An antiserum (no. 314) prepared against apparently intact turnip mosaic potyvirus (TuMV) particles showed a high cross‐reactivity with all of 20 aphid‐borne potyviruses tested as well as the mite‐borne ryegrass mosaic rymovirus (RGMV) and the fungus‐borne barley mild mosaic bymovirus (BaMMV) with two of the four methods used (indirect PTA‐ELISA or IEM and Western blotting). In immunoelectron microscopic decoration, about two thirds of the viruses tested reacted positively whereas in the direct DAS‐ELISA only the homologous TuMV reacted positively. Another antiserum (no. 203) produced by using as immunogen a mixture of the dissociated core proteins of three trypsin‐treated potyviruses (potato A and V and plum pox viruses), reacted positively in both indirect ELISA and Western blotting with all aphid‐borne potyviruses and RGMV. No reactions were obtained with the other two methods. The causes for these different reactivities are discussed. Both antisera are useful for the detection of aphid‐borne potyviruses, but one (no. 314) is especially suitable for the detection of these viruses under routine conditions by use of a simple indirect PTA‐ELISA.