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Agrobacterium tumehciens After in vivo Inoculation of Potato (Sohnum tuberosum L.) (Scanning Electron Microscopy)
Author(s) -
Starkbauerova Zuzana,
ŠRobárová Antonia
Publication year - 1993
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1993.tb01422.x
Subject(s) - xylem , biology , agrobacterium tumefaciens , inoculation , parenchyma , agrobacterium , bacteria , solanum tuberosum , botany , microbiology and biotechnology , transformation (genetics) , horticulture , biochemistry , genetics , gene
Five weeks after the in vivo inoculation of potatoes ( Solanum tuberosum L.) with Agrobacterium. tumefaciens strain B6S3, bacteria were found in the non‐differentiated cells of tumors (formed from xylem parenchyma or other living cells), in xylem cells at the site of inoculation, as well as in xylem cells of the adjacent stem. Bacteria were attached by fibrillar aggregates to the tumor cell walls. They were also attached to a fibrillar mass which arose from agrobacteria connected to this mass in the tumor. Agrobacteria, singly or in pairs, were attached to an electron dense formation (possibly bacterial extracellular polysaccharides) found both inside the xylem cells of the stem adjacent to the tumor and at the site of inoculation. Some A. tumefaciens cells were attached by means of a pedestal‐like structure at the inoculation site. A possible function of the different means of attachment of A. tumefaciens in both nontransformed plant cells and tumors is discussed.