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Interaction between Pseudomonas syringae pv. Pisi and Isolated Pea Mesophyll Protoplasts
Author(s) -
Akpa A. D.,
Archer S. A.
Publication year - 1993
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1993.tb01407.x
Subject(s) - protoplast , pseudomonas syringae , biology , cultivar , botany , pseudomonas , inoculation , pseudomonadaceae , host (biology) , cell wall , bacteria , microbiology and biotechnology , pathogen , horticulture , genetics
The interaction between five races of Pseudomonas syringae pv. pisi (PSP) and isolated mesophyll protoplasts obtained from five pea cultivars was studied. There was no trend in the attachment of bacterial cells to surfaces of compatible and incompatible host protoplasts. The viability of protoplasts from compatible and incompatible host‐pathogen interactions did not differ significantly; however, changes in the viability of cultivar Kelvedon Wonder protoplasts, compatible with all five races, was relatively more stable following inoculation with bacteria than those of cultivar Fortune, incompatible with all of the five races. Protoplast cell wall regeneration did not take place until 24–48 h after isolation. It is concluded that the use of pea protoplasts as a model system for studying the pea‐PSP interaction appears to have considerable potential for the future, but more basic research is required.