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Restriction Fragment Length Polymorphisms of Mitochondrial and Nuclear DNAs among Korean Races of Magnaporthe grisea
Author(s) -
Ko Jong Hyun,
Hwang Byung Kook,
Hwang Byung Gap
Publication year - 1993
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1993.tb01359.x
Subject(s) - magnaporthe grisea , biology , restriction fragment length polymorphism , mitochondrial dna , nuclear dna , genetics , southern blot , restriction enzyme , japonica , virulence , dna , gene , oryza sativa , botany , polymerase chain reaction
Three KJ‐races of Magnaporthe grisea (virulent to only japonica type rice cultivars) and seven KI‐races (virulent to either indica or japonica type cultivars) isolated from various rice‐growing areas of Korea were assayed for restriction fragment length polymorphisms (RFLPs) in mitochondrial and nuclear DNAs of the fungus. The size of mitochondrial DNA of M. grisea was estimated to be approximately 39. 8 kb. No RFLP in the mt DNA was detected among the 10 Korean races, indicating an extensive conservation in the gene composition of mt DNA without sequence variation. The identical restriction patterns of mt DNA also suggest that mt DNA of M. grisea may not be responsible for pathotypic diversity and variability. Southern blot analysis with five single‐copy DNA probes showed considerable polymorphisms. Much diversity was detected in the three KI‐races predominated during 1978–1985 in Korea. In contrast, no genetic variation was detected between other four KI‐races and three KJ‐races. RFLPs in nuclear DNA were correlated to some extent with the prevailing races in Korea. However, relationship between RFLPs in nuclear DNA and virulence of M. grisea races was inconclusive.