Premium
Ultrastructure in Bean Leaves Infiltrated with Bacterial Extracellular Polysaccharides
Author(s) -
Stefani E.,
Medeghini Bonatti P.
Publication year - 1993
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1993.tb01339.x
Subject(s) - ultrastructure , chloroplast , thylakoid , biology , stroma , extracellular , cytoplasm , vacuole , periplasmic space , plastid , cell wall , chloroplast membrane , chloroplast stroma , botany , palisade cell , vesicle , microbiology and biotechnology , biophysics , biochemistry , membrane , immunohistochemistry , escherichia coli , immunology , gene
Ultrastructural observations were carried out in bean leaf tissue infiltrated with bacterial extracellular polysaccharides. These investigations revealed changes m the cell fine structure, especially related with chloroplast organization. Up to 24 h after EPS infiltration alterations consisted in irregularly running thylakoids and distortions of stacked regions. Invaginations of the plastid envelope and appearance of cytoplasm pockets inside the stroma were also noted. The most severe alterations, consisting in stroma dilations and envelope infoldings, were noted 24 h after EPS infiltration, parallehng the yellowing of the treated leaf areas; chloroplast shrinkage and collapse of thylakoid system were also sometimes observed. Chloroplast ultrastructure generally recovered 48 h after treatment; at this time local detachment of the plasma membrane and vesicle formation in the periplasmic space were observed, resembling a non specific, locahzed cellular response. The observed, permanent chlorosis and ultrastructural alterations suggest an interference of the EPS in the global metabolism of the bean mesophyll cell.