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Purification and Some Properties of an Isolate of Beet Yellows Virus from Ukraine
Author(s) -
Rogov V. V.,
Karasev A. V.,
Agranovsky A. A.
Publication year - 1993
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1993.tb01328.x
Subject(s) - biology , sugar beet , antiserum , myzus persicae , plant virus , virus , horticulture , virology , luteovirus , botany , sucrose , antibody , food science , aphid , immunology
A purification procedure, which yielded up to 15–30 mg of beet yellows virus (BYV) per 100 g of infected Tetragonia expansa leaves, has been developed. The procedure included sap clarification with Triton X‐100, and two cycles of ultracentrifugation through sucrose cushion, which contained PEG‐6000 and NaCl. A specific antiserum was prepared, and BYV infection was successfully detected by the double‐antibody sandwich (DAS) ELISA in infected sugar beet leaves and roots diluted up to 1 × 10 5 and 1 × 10 4 , respectively. The virus concentration was demonstrated to decrease in infected sugar beet roots slowly during 7 months, thus allowing successful diagnosis of planting material in winter storage. BYV presence in Myzus persicae aphids was also reliably detectable using the DAS‐ELISA. In a competitive DAS‐ELISA test, the Ukraine and the British BYV isolates were found serologically indistinguishable.