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Cytopathology of Lettuce Mosaic Virus‐infected Lettuce Seeds and Seedlings
Author(s) -
Hunter D. G.,
Bowyer J. W.
Publication year - 1993
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1993.tb01326.x
Subject(s) - biology , cotyledon , endosperm , immunogold labelling , radicle , ultrastructure , botany , cytochemistry , hypocotyl , potyvirus , plasmodesma , plant virus , germination , virus , virology
The ultrastructure of lettuce mosaic potyvirus (LMV)‐infected lettuce seeds and seedlings was studied by transmission electron microscopy. Conventional thin‐section electron microscopy and immunogold cytochemistry were both successfully employed to study the location of LMV in embryonic and non‐embryonic seed parts. LMV particle aggregates and cytoplasmic “pinwheel” inclusions characteristic of potyviruses were observed throughout the embryonic tissues (radicle, hypocotyl and cotyledon) of infected lettuce seeds and seedlings, and also in the non‐embryonic endosperm layer. LMV particles, but not inclusions, were also located in the non‐embryonic pericarp layer.