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A Detached Cotyledon Test for the Isolation of Mutants of Pyrenopeziza brassicae Defective in Pathogenicity Determinants
Author(s) -
Ball A. M.,
Ingram D. S.,
Johnstone K.
Publication year - 1992
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1992.tb01299.x
Subject(s) - biology , cotyledon , pathogenicity , conidium , blackleg , spore , brassica , botany , mutant , inoculation , pathogen , microbiology and biotechnology , horticulture , genetics , gene
A detached cotyledon pathogenicity test was devised for the isolation of UV‐induced mutants of the hemibiotrophie ascomycete pathogen of Brassica spp., Pyrenopeziza brassicae defective in pathogenicity determinants. At least 95 % of cotyledons of suitable susceptible cultivars of oilseed rape ( Brassica napus L. ssp. oleifera cvs Shogun or Bolko) showed symptoms of disease (white spore pustules [conidiomata] on the surface of the cotyledon) within three weeks of inoculation of cotyledons with 10 4 conidia of P. brassicae. The test allowed rapid screening of UV‐induced mutants with a low frequency of false negatives. From 1,700 survivors of UV mutagenesis tested, 20 non‐pathogenic mutants were obtained.