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The Isolation and Evaluation of Two Naturally Occurring Mild Strains of Vanilla Necrosis Potyvirus for Control by Cross‐Protection
Author(s) -
Liefting Lia,
Pearson Michael,
Pone Semisi
Publication year - 1992
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1992.tb01276.x
Subject(s) - nicotiana benthamiana , potyvirus , biology , inoculation , virology , mottle , titer , necrosis , isolation (microbiology) , virus , plant virus , microbiology and biotechnology , horticulture , genetics
In an attempt to find mild virus strains that would cross‐protect sgainst vanilla necrosis potyvirus (VNPV), Vanilla fragrans plants in Tonga were surveyed for the presence of mild or symptomless potyvirus infections. Potyviruses were detected by indirect ELISA using a commercially available portyvirus group monoclonal anibody. From 28 plants with mild or symptomless infections two portyvirus isolates, designated V1 and V3, included systemic infections in Nicotiana benthamiana following mechanical inoculation. V1, which causes a mild mottle in N. benthamiana , is serologically related to VNPV, while V3 which causes mild vein banding is serologically unrelated to VNPV. Prior inoculation with V1 protected N. benthamiana against the severe mosaic symptoms of VNPV when challenge inoculated after 14 and 21 days, but not after 7 days. When V3 was used as the protecting strain, cross‐protection was observed in some, but not all plants, when chalenged with VNPV after 14 and 21 days.