Premium
Artificial Induction and Evaluation of a Mild Isolate of Tomato Spotted Wilt Virus
Author(s) -
Wang Min,
Gonsalves Dennis
Publication year - 1992
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1992.tb01271.x
Subject(s) - datura stramonium , lycopersicon , biology , lactuca , inoculation , horticulture , solanaceae , nicotiana tabacum , datura , botany , biochemistry , gene
A severe isolate (BL) of tomato spotted wilt virus (TSWV) that originated from Hawaii was treated with nitrous acid in an effort to obtain mild mutants. The standardized procedure used in mutation experiments was: extracting infected Gomphrena globosa L. leaf tissue in 0.01 M Na 2 SO 3 , 0.125 M sodium acetate and 0.4 M sodium nitrite at pH 5.5 and incubating the extract for 20 min at room temperature. The extract was inoculated to tobacco ( Nicotiana tabacum L. cv. Havana 423) and local lesions were subsequently transferred to lettuce ( Lactuca sativa L. cv. Minetto). One isolate (R27G) that incited mild symptoms in lettuce was obtained out of 868 local‐lesion‐transfers. Under greenhouse conditions, the isolate induced mild symptoms on tomato ( Lycopersicon esculentum Mill.) but was severe on peppers ( Capsicum annuum L.). The effect of the R27G isolate on growth of potted tomatoes kept outdoors was variable. In one trial, only 15 % of the fruit had symptoms versus 67 % in another trial. R27G fully protected Datura stramonium L. plants that were challenge inoculated with the severe parent BL isolate. Less effective cross protection was observed against a severe isolate from Oklahoma.