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Cell Surface Properties of Pseudomonas syringae pv. phaseolicola Wild‐type and hrp Mutants
Author(s) -
Fett WilliamF.,
Osman StanleyF.,
Dunn MichaelF.,
Panopoulos NickolasJ.
Publication year - 1992
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1992.tb01260.x
Subject(s) - pseudomonas syringae , mutant , biology , pseudomonadaceae , polysaccharide , wild type , strain (injury) , microbiology and biotechnology , lipopolysaccharide , pseudomonadales , cell wall , glycolipid , pseudomonas , bacteria , biochemistry , gene , pathogen , genetics , anatomy , endocrinology
The cell surface hydrophobicity and charge as well as surface polysaccharides of eight independent prototrophic hrp ::‐Tn 5 mutants (L indgren et al. , J. Bacteriol. 168 , 512–522, 1986) were compared to the wild‐type parent strain NPS3121 of Pseudomonas syringae pv. phaseolicola . No significant differences were found in cell surface charge, but mutant strain NPS4005 exhibited significantly lower cell surface hydrophobicity than the wild‐type and the other mutant strains. The mutant strains all retained the ability to produce the exopolysaccharides (EPS) levan, a neutral fructan, and alginate, an acidic polymer. Relative amounts of EPS produced in vitro was dependent on culture conditions. Lipopolysaccharide (LPS) chemotypes were similar for all nine strains. Chemical as well as 13 C‐NMR analyses of the O‐antigens from four wild‐type strains of P. s. pv. phaseolicola representing two physiological races as well as the O‐antigens of two strains of P. s. pv. syringae which belong to the same serogroup as P. s. pv. phaseolicola indicated that all of the O‐antigens were very similar if not identical. LPS of three strains of P. s. pv. phaseolicola produced in vitro or in planta were also compared and no significant differences were detected. The altered phenotype of the Tn 5 mutants of P. s. pv. phaseolicola does not appear to be due to changes in the ability to produce exopolysaccharides or to an altered composition of cell surface polysaccharides (LPS and EPS). However, a change in an unidentified cell surface component(s) leading to lowered cell surface hydrophobicity of mutant strain NPS4005 may be important.