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Some Characteristics of Potyvirus Isolates from Vanilla tahitensis in French Polynesia and the Cook Islands
Author(s) -
Wang Y.Y.,
Pearson M. N.
Publication year - 1992
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1992.tb01252.x
Subject(s) - potyvirus , biology , antiserum , plant virus , host (biology) , polyclonal antibodies , virology , botany , mottle , virus , antibody , genetics
Abstract Further characterization of vanilla mosaic potyvirus (VaMV) confirmed that it is quite distinct from vanilla necrosis potyvirus (VNV). In immuno‐blot ELISA, a polyclonal antiserum to vanilla mosaic virus from Vanilla tahitensis in French Polynesia did not react with vanilla necrosis potyvirus infected V. fragrans from Tonga. However, samples of V. tahitensis with leaf distortion and mosaic from the Cook Islands reacted strongly with the VaMV antiserum. Host range studies involving 20 plant species failed to find a host for VaMV outside the genus Vanilla , confirming that VaMV has a much more restricted host range than VNV. The mean particle length of V a MV from purified preparations was 763 nm (Std. Dev. 48) and SDS‐polyacrylamide electrophoresis indicates a single coat polypeptide of M r 34 × lO 3 .