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Simultaneous Detection of Several Nepoviruses Infecting Grapevine in a Single DAS‐ELISA Test Using Mixed Antisera
Author(s) -
Etienne L.,
Clauzel J. M.,
Fuchs M.
Publication year - 1991
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1991.tb04735.x
Subject(s) - biology , antiserum , virology , serotype , polyclonal antibodies , nepovirus , microbiology and biotechnology , antibody , plant virus , virus , genetics
Single and mixed antisera have been compared in DAS‐ELISA for the routine diagnosis of nepoviruses infecting grapevine. The use of mixed polyclonal antibodies allowed in a single test the simultaneous detection of several nepoviruses (ArMV + GFLV) or serotypes of nepoviruses (TBRV serotypes G + S and RRV serotypes E + G) whatever the nature of the antigens, e.g. purified virions, diseased grapevine leaves or grapevine wood shavings. The detection was as reliable and efficient as with simple antibodies. The plant samples which were positively diagnosed by mixed antisera often showed an increase of their absorbance values, in comparison with the detection using simple antisera, while the background level was unchanged. The origin of this enhancement remains unclear, but it seems to be closely related to the mixing of the conjugated antibodies.