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Differentiation of the Soft‐Rotting Erwinias (the Carotovora Group) by Fatty Acid Composition
Author(s) -
Wells J. M.,
Moline H. E.
Publication year - 1991
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1991.tb04567.x
Subject(s) - biology , fatty acid , erwinia , composition (language) , bacteria , subspecies , biochemistry , food science , gene , ecology , genetics , philosophy , linguistics
About 90 % of total cellular fatty acids in E. carotovora , grown on KB medium for 1 day at 28°C, were the saturated, even‐carbon straight chains 12 : 0 (5.9%), 14 : 0 (1.7%) and 16 : 0 (30.1 %), and the unsaturated 16 : 1 (36.6%) and 18 : 1 (15.0%) fatty acids. Other components were the hydroxy‐substituted 3‐OH 14 : 0 (5.3%) and 21 minor fatty acids each occurring less than 0.1 % of the total — 14 of them reported herein for the first time in Erwinia . The ratio of 16 : 1/18 : 1 in KB‐grown cells was as useful in differentiating subspecies of E. carotovora as previously reported by other workers for TSA‐grown cells. A comparison of fatty acid profiles of E. carotovora on 4 different media, KB, TSA, NA and PDA, indicated that on KB there was the greatest proportion of Class A and C fatty acids, and the highest number of detectable components. Significant differences were noted in the 5 major fatty acids and in cyclic fatty acids among the 4 species of the carotovora group – E. carotovora, E. chrysanthemi, E. rhapontici and E. cypripedii . These differences could be expressed as algorithms that, when used in sequential dichotomous steps, could differentiate the 4 species.