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Inorganic Pyrophosphatase from E. coli as a Label for the Detection of Plant Viruses by ELISA
Author(s) -
Mizenina O. A.,
Borisova O. V.,
Novikov V. K.,
Evtushenko O. A.,
Baykov A. A.,
Atabekov J. G.
Publication year - 1991
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1991.tb00163.x
Subject(s) - pyrophosphatase , inorganic pyrophosphatase , pyrophosphate , immunoassay , biology , substrate (aquarium) , conjugated system , enzyme , plant virus , biochemistry , substrate specificity , antibody , microbiology and biotechnology , chromatography , virology , virus , chemistry , organic chemistry , polymer , ecology , immunology
Inorganic pyrophosphatase (PPase) from E. coli was used as label in enzyme immunoassay for the detection of different plant viruses. The use of PPase‐conjugated antibodies and tetrasodium pyrophosphate as a substrate in ELISA provides some advantages in detection of plant viruses in purified preparations and extracts from plant specimens: high sensitivity, negligible level of background reactions in control samples, bright blue‐greenish colour which allows to detect the reaction visually and high stability of PPase‐conjugated antibodies.