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Consecutive and Simultaneous Competition ELISA of Monoclonal Antibodies: Characterization of Epitope Relationships or Immunoglobulin Idiotypes
Author(s) -
Kubanek C. A.,
Hill J. H.,
Van Deusen R. A.,
Durand D. P.
Publication year - 1991
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1991.tb00142.x
Subject(s) - epitope , monoclonal antibody , biology , biotinylation , antibody , antigen , virology , competition (biology) , microbiology and biotechnology , immunology , ecology
A panel of monoclonal antibodies prepared against cowpea severe mosaic virus was divided into two groups based upon presence or absence of competition with a biotinylated antibody in competition ELISA. Antibodies that did compete could be further characterized by comparison of competition curves obtained from assays performed in simultaneous and consecutive manners. These two types of competition assays provide an easy method to define intially relationships among epitopes on antigens and to characterize specific immunoreactivity of different antibodies to the antigen.