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Identification of Peanut Green Mosaic Virus Strains in India *
Author(s) -
Naidu R. A.,
Sreenivasulu P.,
Manohar S. K.,
Ramakrishna K. N.,
Reddy D. V. R.,
Nayudu M. V.
Publication year - 1991
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1991.tb00136.x
Subject(s) - biology , antiserum , mottle , virus , virology , mosaic virus , tobacco mosaic virus , soybean mosaic virus , plant virus , cowpea mosaic virus , alfalfa mosaic virus , potyvirus , potato virus y , coat protein , antibody , rna , gene , genetics
During field surveys, three peanut green mosaic virus isolates differing in symptomatology on groundnut and a few other hosts were collected. Ultrathin sections of infected groundnut leaflets showed cytoplasmic inclusions with pin wheels and scrolls. In enzyme‐linked immunosorbent assay they reacted strongly with antisera to peanut green mosaic and soybean mosaic virus antisera, and moderately with adzuki bean mosaic and peanut stripe virus antisera. All isolates also reacted positively with antisera to peanut eye spot, blackeye cowpea mosaic, pea seed‐borne mosaic, potato virus Y and tobacco etch viruses, and did not react with antisera to peanut mottle, bean yellow mosaic, bean common mosaic, clover yellow vein and sugarcane mosaic viruses. SDS‐PAGE analysis of purified virus preparations of the three isolates showed a single polypeptide with mol. wt. of 34,500 daltons. Based on these results, the three isolates are identified as biologically distinct strains of peanut green mosaic virus.