Premium
Evaluation of Tip Culture, Thermotherapy and Chemotherapy for Elimination of Peanut Mottle Virus from Arachis hypogaea
Author(s) -
Chen W. Q.,
Sherwood J. L.
Publication year - 1991
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1991.tb00115.x
Subject(s) - arachis hypogaea , biology , mottle , virology , virus , horticulture , plant virus
Peanut ( Arachis hypogaea L.) plants (cvs Florunner and Pronto) were inoculated at the two‐leaf stage with peanut mottle virus (PMV) to obtain PMV‐infected plants. Shoot‐tips from plants grown in the glasshouse (27°C) or from plants maintained at 35°C were used for tip culture. In some experiments ribavirin was added to the culture medium at 5 mg/1, 10 mg/1, 15 mg/1 or 20 mg/1. Plants regenerated from meristems or shoot‐tips taken from virus‐infected plants were not virus‐free. After 45 days at 35°C, foliar tissue of 93 % of Florunner and 95 % of Pronto plants tested negative for PMV by enzyme‐linked immunosorbent assay (ELISA). When shoot‐tips from the plant that tested negative by ELISA were used for tip culture, no virus‐free plants were obtained. No virus‐free plants were obtained from tips cultured on medium supplemnted with ribavirin. However, when tip culture, thermotherapy and chemotherapy were combined; 80 % of Florunner and 100 % of Pronto plants were found negative for PMV.