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Comparison of Polyclonal Antisera in the Detection of Tomato Spotted Wilt Virus Using the Double Antibody Sandwich and Cocktail ELISA
Author(s) -
Resende R.deO.,
Avila A. C.de,
Goldbach R. W.,
Peters D.
Publication year - 1991
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1991.tb00092.x
Subject(s) - antiserum , polyclonal antibodies , biology , serial dilution , virology , virus , tospovirus , antibody , antigen , tomato spotted wilt virus , microbiology and biotechnology , plant virus , immunology , medicine , alternative medicine , pathology
Different polyclonal antisera and enzyme‐linked immunosorbent assay (ELISA) procedures have been tested for their potential to detect tomato spotted wilt virus (TSWV). The virus could efficiently be detected in high dilutions of sap from infected plants, and at low concentrations of purified virus and nucleocapsid protein preparations in the cocktail ELISA and the double antibody sandwich ELISA (DAS‐ELISA). Amounts of 1 to 3 ng of virus protein still gave positive readings using purified preparations, while sap could be diluted approximately 100,000 times. Differences in the detection level were observed using nucleocapsid protein antiserum (anti‐N‐serum) and the antiserum against intact virus particles (anti‐TSWV‐serum), but both antisera showed to be powerful sera for the detection of TSWV. Using anti‐N‐serum, TSWV could be detected in highly diluted extracts of different hosts, and also in leaf extracts or intact tissues stored for 30 days under different conditions. These results indicate that the TSWV nucleocapsid protein remains antigenic for long periods.