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Potato Protoplast‐derived Callus Tissue Challenged with Erwinia carotovora subsp. carotovora: Survival, Growth and Identification of Resistant Callus Lines *
Author(s) -
Taylor RaymondJ.,
Secor GaryA.
Publication year - 1990
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1990.tb04589.x
Subject(s) - callus , inoculation , biology , erwinia , pathogen , protoplast , bacteria , tissue culture , murashige and skoog medium , botany , microbiology and biotechnology , horticulture , in vitro , biochemistry , genetics
Potato (cv. Crystal) protoplast‐derived callus tissue was evaluated for survival and growth when exposed to Erwinia carotovora subsp. carotovora (strain Ecc71). Calli were either directly exposed to the pathogen by inoculation or to metabolites produced by the pathogen via a bilayer medium. Individual calli were inoculated with 0.5 μl of bacterial suspensions at 10 4 , 10 5 , 10 6 , 10 7 , 10 8 and 10 9 cfu/ml. The bilayer consistedof 10 ml of callus proliferation medium supplemented with pectin (2 g/l) and contained bacteria at 10 2 , 10 3 , 10 4 , 10 5 and 10 6 cfu/ml. This medium was overlaid with 10 ml of bacteria free callus induction medium. Mean callus diameter of the inoculated treatments increased for 24 h, then declined. Over 90% of the inoculated calli were killed within 5 days but some survived as long as 14 days. Calli grown on the bilayer medium containing 10 6 , 10 5 and 10 4 cfu/ml also decreased in size. Most were killed within 9 days but some survived 20 days. Calli exposed to 10 3 and 10 2 cfu/ml experienced limited growth with 20% and 7%, respectively, surviving after 27 days. Reactions to the pathogen varied considerably within the callus populations and individual calli with extended survival were identified in both experiments.