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Specificity of Antisera Against Rhodococcus fascians (Tilford) Goodfellow in Indirect Immunofluorescence
Author(s) -
Scortichini M.,
Todisco C.,
Varvaro L.
Publication year - 1990
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1990.tb04309.x
Subject(s) - antiserum , biology , titer , indirect immunofluorescence , microbiology and biotechnology , immunofluorescence , bacteria , erwinia , virology , antibody , immunology , genetics , virus
The possibility to obtain specific antisera to detect Rhodococcus fascians in imported lily bulbs is taken into consideration for not allowing the admission of this pathogen in Italy. For the production of specific antisera and in order to avoid the occurtence of cross‐reactions between R. fascians and some of the most widespread soilborne, plant decay bacteria and with Erwinia carotovora subsp. carotovora a preliminary work for singling out the best immunogens, scheduleof immunization and antisera dilution for using in indirect immunofluorescence has been carried out. Living cells and heattreated cells were proved to be good immunogens and long‐term immunization provided higher titers than short‐term immunization. Toobtain a satisfactory specificity the dilution of the antisera is required. The 1: 800 dilution is quite effective in overcoming cross‐reactions whereas undiluted antisera and antisera used at 1: 100 and 1: 200 dilution did not provide specificity.