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A Rapid Viability Test for Sclerotia with Fluorescein Diacetate
Author(s) -
Dittmer U.,
Weltzien H. C.
Publication year - 1990
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1990.tb01153.x
Subject(s) - acridine orange , staining , biology , fluorescein , orange (colour) , fluorescence , chromatography , microbiology and biotechnology , horticulture , chemistry , genetics , physics , quantum mechanics
Four different fluorescent dyes Acridine Orange (AO), Fluorescein Diacetate (FDA), Calcofluor White M2R (CW), and Europium (III) Thenoyltrifluoroacetonate, 3‐Hydrate (Eu(TTA) 3 ) were tested to distinguish rapidly between dead and living sclerotia from Sclerotinia trifoliorum. FDA, 0.01% diluted in phosphate buffer, had the shortest staining period and was easy to handle. It is especially suitable to test large numbers of sclerotia for viability. Furthermore the preparation of the samples for the staining procedure is described in this paper.