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Base Composition of the DNA of Mycoplasma‐Like Organisms Associated with Various Plant Diseases
Author(s) -
Kollar A.,
Seemüller E.
Publication year - 1989
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1989.tb01127.x
Subject(s) - phyllody , biology , virescence , dna , buoyant density , mycoplasma , aster yellows , botany , differential centrifugation , host (biology) , microbiology and biotechnology , biochemistry , genetics , polymerase chain reaction , gene , restriction fragment length polymorphism
DNA was isolated from periwinkle plants infected by various mycoplasma‐like organisms (MLOs), and from apple trees affected by apple proliferation. The DNA of the causal agents was separated from the host plant DNA by repeated bisbenzimide‐CsCl buoyant density gradient centrifugation which resulted in highly enriched MLO DNA bands characterized by a lower buoyant density than that of the host DNA. The MLO DNAs were hydrolyzed to free bases which were determined by HPLC. The analyses revealed a similar low G + C content as found in the DNAs of several culturable mycoplasmas and spiroplasmas. The values of the DNA of the agents of the diseases investigated were as follows: European aster yellows 23.0, periwinkle virescence 23.5, apple proliferation 23.7, rape virescence 24.2, and phyllody of Diplotaxis erucoidcs 26.2 mol % G + C, respectively. Methylated bases were detected in low amounts only.