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In vitro Culture of Grapevine Infected by Closterovirus Type III *
Author(s) -
Barba M.,
Cupidi A.,
Faggioli F.
Publication year - 1989
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1989.tb01108.x
Subject(s) - closterovirus , biology , herbaceous plant , cutting , horticulture , in vitro , plant virus , botany , virus , virology , biochemistry
Herbaceous cuttings collected in autumn from a closterovirus type III infected grapevine plant (cv. Merlot) were micropropagated in vitro . Results showed thai cultured plants did not present any leaf symptoms even if they grow slower than healthy control plants by a ratio of about 1/2. The virus concentration, as estimated by electron microscopy, was found constant when observed fortnightly over a period of 75 days. The number of particles was about 30 times higher than in viral preparations obtained from infected material collected in the field.