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ELISA Detection of Odontoglossum Ringspot Virus in Mature Plants and Protocorms of Cymbidium Orchids: Potential Solutions to Problems of Sample Preparation Time and Low Virus Concentration
Author(s) -
Arunasalam G.,
Pearson M. N.
Publication year - 1989
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1989.tb01099.x
Subject(s) - cymbidium , biology , virus , relative standard deviation , antiserum , virology , orchidaceae , horticulture , detection limit , botany , chromatography , chemistry , antibody , immunology
The ability to detect Odontoglossum ringspot virus (ORSV) released from the cut surfaces of leaf discs by ELISA was examined. Results indicate that ORSV from leaf discs can be detected but that multiple discs are necessary to obtain reliable detection of low virus concentrations, ELISA of Cymbidium protocorms, known from immunosorbent electron microscopy to be infected with ORSV, frequently produced A 435 values which would be considered either negative or marginally positive on the basis of commonly accepted statistical limits (i, e. 2 x mean, or mean + 3 x standard deviation). The comparison of paired samples, one of which had been pretteated with ORSV antiserum, improved the sensitivity of the test from 10 ng ml ‘’ of virus to 2 ng ml’.