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A Preparation Method of Aspergillus spp. for Scanning Electron Microscopy
Author(s) -
Weidenbornur M.,
Uzih A.,
Hamacher J.,
Hindori H.,
Weltzitzien H. C.
Publication year - 1989
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1989.tb01083.x
Subject(s) - mycelium , vapours , osmium tetroxide , aspergillus flavus , biology , scanning electron microscope , fixation (population genetics) , electron microscope , botany , materials science , biochemistry , composite material , physics , neuroscience , gene , optics
For scaning electron microscopy (SEM) of Aspergillus flavus Link und A. petraku Vörös. Simultaneous fixation and drying in osmium tetroxide (O 5 O 4 vapours was superior to the conventional preparation methods. Which involve critical point drying. Mycelium exposed to vapours from a solution of 2% O 5 O 4 for 24 h at ca. 20°C retained its turger and natural structures. Best results were achived when young mycelium was used. whereas old mycelium always collapased, irrespective of the fixation method used. Use of this method for fixation of Aspergillus spp. is therefore preferable to convennonal methods which are laborious. time‐consuming and often interfere with the natural habit of structures. It may also prove usefull with other fungal structures.