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Rapid Immunofluorescent Detection of the Grapevine Flavescence Doree Mycoplasmalike Organism in the Salivary Glands of the Leafhopper Euscelidius variegates Kbm
Author(s) -
Lherminier J.,
Terwisscha van Scheltinga T.,
BoudonPadieu E.,
Caudwell A.
Publication year - 1989
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1989.tb01080.x
Subject(s) - biology , polyclonal antibodies , staining , saliva , leafhopper , virology , vector (molecular biology) , microbiology and biotechnology , botany , antibody , immunology , recombinant dna , biochemistry , genetics , gene , hemiptera
Polyclonal rabbit antibodies, prepared against partially purified mycoplasmalike‐organisms (MLO) from Grapevine Flavescence Dorée‐infected plant extracts, were used to detect MLO in situ in excised salivary glands of the experimental vector Euscelidius variegates Kbm by an indirect immunofluorescent staining technique. Glands were examined from the fourth to the tenth week after the start of MLO acquisition. MLO detection was compared using this method, the transmission test and the ELISA technique applied to insects corpses after removal of salivary glands.