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Occurrence and Identification of Eggplant Mottled Crinkle Virus in India
Author(s) -
Raj S. K.,
Aslam M.,
Srivastava K. M.,
Singh B. P.
Publication year - 1989
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1989.tb01072.x
Subject(s) - biology , antiserum , capsid , virus , solanum , virology , polyacrylamide gel electrophoresis , serology , titer , alfalfa mosaic virus , plant virus , gel electrophoresis , strain (injury) , coat protein , botany , microbiology and biotechnology , antibody , gene , biochemistry , enzyme , genetics , rna , anatomy
A virus isolated in India from eggplant ( Solanum melongena ) showing chlorotic rings, line pattern and mosaic on leaves accompanied with stunted growth was identified as a strain of eggplant mottled crinkle virus on the basis of host range, in vitro properties, particle morphology, molecular weight of capsid protein and serological relationship. The sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of virus coat protein revealed the presence of a single polypeptide with a molecular weight of 41,000. An antiserum to the virus produced in rabbit had a titre of 1: 2048 in double diffusion tests.