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Direct Introduction of Fluorescein Isothiocyanate‐conjugated Albumin into Intact Macroconidia of Fusarium oxysporum f. sp. lycopersici by Electroporation
Author(s) -
Matsuda Y.,
Toyoda H.,
Nishiguchi T.,
Ouchi S.
Publication year - 1989
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1989.tb01059.x
Subject(s) - fluorescein isothiocyanate , fluorescein , biology , fusarium oxysporum , electroporation , spore , conidium , microbiology and biotechnology , albumin , conjugated system , hypha , fluorescence , botany , biochemistry , chemistry , polymer , physics , organic chemistry , gene , quantum mechanics
An electroporation procedure for the introduction of fluorescein isothiocyanate‐conjugated albumin into intact macroconidia of Fusarium oxysporum f. sp. lycopersici was performed in this paper. FITC‐albumin was used to establish an efficient electroporation procedure because its presence in spores could be easily detected by fluorescence microscopy. The uptake of fluorescein isothiocyanate‐conjugated albumin into spores was successfully mediated by high voltage electrical pulses at field strength of 10 KV/cm. In electroporation of 10 6 macroconidia per ml with the present system, 1.2 × 10 5 spores incorporated the protein, and then 3.1 × 10 4 normally germinated, elongated vegetative hyphae, and produced spores.