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The Seed‐borne Inoculum of Peronospora manshurica , Causal Agent of Soybean Downy Mildew
Author(s) -
Roongruangsree UTai,
Olson L. W.,
Lange L.
Publication year - 1988
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1988.tb04473.x
Subject(s) - mycelium , oospore , biology , downy mildew , feulgen stain , botany , germination , ascocarp , spore , staining , horticulture , taxonomy (biology) , genetics
The seed‐borne inoculum of P. manshurica is described using various microscopic techniques and staining reactions. The inoculum, in addition to the oospores, consists of a thin walled mycelium under the hourglass cell layer of the seed coat and a thick walled resting mycelium in the oospore crust on the seed surface. By a modified Feulgen staining technique it was possible to demonstrate that the thin walled and the thick walled resting mycelium as well as thin and thick walled oogonia contain nuclei which are Feulgen positive suggesting that portions of the mycelium of P. manshurica may survive even on the surface of dry, mature seeds.