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Purification and Electron Microscopy Studies of a Probable Potyvirus from Polianthes tuberosa L.
Author(s) -
Horner MaryB.,
Pearson M. N.
Publication year - 1988
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1988.tb01015.x
Subject(s) - potyvirus , polianthes tuberosa , biology , potyviridae , mottle , electron microscope , botany , virus , turnip mosaic virus , plant virus , horticulture , virology , physics , optics
A partial purification procedure is described for a flexuous filamentous virus from leaves of the ornamental crop tuberose ( Polianthes tuberosa L.) exhibiting mottle symptoms. Particular problems associated with the purification included particle aggregation and the highly viscous nature of the plant sap. The former difficulty was partially alleviated by the addition of 1.0 M urea to the extraction buffer and the latter by treatment of the extract with cellulase. The mean virus particle length from negatively stained leaf dips was 742 nm (SD = 54) which is characteristic of viruses belonging to the potyvirus group. In addition, electron microscopy of thin sections of infected leaf tissue demonstrated the presence of several types of inclusion bodies similar in appearance to inclusions previously described in association with potyvirus infections in other plants.