Premium
RNA/cDNA Hybridization and Infectivity Tests Suggest that Barley Yellow Mosaic Virus Isolate M has a Bipartite Genome
Author(s) -
Koenig R.,
Huth W.
Publication year - 1988
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1988.tb00982.x
Subject(s) - biology , subgenomic mrna , genome , rna , virology , complementary dna , genetics , genomic organization , mosaic virus , virus , infectivity , gene , plant virus , microbiology and biotechnology
Northern blot analysis with random‐primed cDNAs revealed that RNAs 1 and 2 of barley yellow mosaic virus (Ba YMV) isolate M have no extensive base sequence homologies. Both RNAs are needed for infection. RNA 2 is therefore neither a subgenomic nor a, satellite RNA, but rather an essential part of the viral genome. Ba YMV isolate M has thus a bipartite genome.