Premium
Small RNAs Originating from Symptomless and Damaged Spruces ( Picea abies L., Karst.) II.
Author(s) -
Köster S.,
Beuther E.,
Riesner D.
Publication year - 1988
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1988.tb00973.x
Subject(s) - picea abies , rna , biology , karst , nucleic acid , nucleotide , botany , horticulture , genetics , gene , paleontology
A nucleic acid of about 450 nucleotides, called RNA 450 , was extracted from needles of symptomless and damaged spruces ( Picea abies L., Karst.) and was analysed by two‐dimensional gel electrophoresis. The samples originated from 14‐years‐old spruces of two differently exposed forest sections of the same location in the Hils area. Trees of section 65 were symptomless whereas those of section 103 were retarded in their growth and had partly yellow coloured needles. A new tree was selected for each sampling. Samples were taken at monthly intervals from June 1985 to March 1986, and bimonthly from March to December 1986. The aim of the study is to find out whether the RNA 450 indicates the damage of trees in the Hils. The RNA 450 was characterized in the accompanying paper (B euther et al. 1988) as an endogeneous RNA, which is influenced in its concentration by tree damages. A comparison of the RNA patterns of needle extracts from four different needle year classes of symptomless and damaged trees led to the result that the concentration of RNA 450 is influenced by season, needle age, and damage. The amplitude of the seasonal changes in the RNA 450 level was found to be larger in younger than in older needles and is not influenced by the position of the branch. The period of metabolic activity, as observed on the level of RNA 450 , is markedly shorter in damaged than in symptomless trees. The usefulness of the RNA 450 as a diagnostic test system will be discussed.