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Small RNAs Originating from Symptomless and Damaged Spruces ( Picea spp.)
Author(s) -
Beuther E.,
Köster S.,
Loss P.,
Schumacher J.,
Riesner D.
Publication year - 1988
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1988.tb00972.x
Subject(s) - rna , biology , botany , horticulture , genetics , gene
Ribonucleic acids were extracted from needles of symptomless and damaged spruces ( Picea spp.). Trees from three locations of different altitudes in Nordrhein‐Westfalen were taken for sampling. The samples were collected from the same individual trees at different times during the growing seasons of the years 1984–1986. Small RNAs (M r <300 000) were analyzed by a two‐dimensional gelelectrophoresis. The aim was to detect “disease associated” RNAs by a comparison of the RNA‐patterns of symptomless and damaged spruces. A RNA of about 450 nucleotides was a strong candidate, though it was found in needles of both damaged and symptomless trees. The concentration of the RNA 450 is dependent on the season, the needle age, and the location of the tree. In damaged trees the level of the RNA 450 ishigher than in symptomless trees. The RNA 450 was found to be an endogeneous RNA, which indicates both the developmental state of the needle, and an interference with the development by the damage already at the early level of RNA metabolism.