Premium
Detection of Tomato Ringspot Nepovirus and a Clostero‐like Virus in French Hybrid Vidal 256 Grapevines
Author(s) -
Podleckis E. V.,
Corbett M. K.
Publication year - 1987
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1987.tb04437.x
Subject(s) - closterovirus , biology , inoculation , virus , virology , botany , nepovirus , plant virus , horticulture , mottle , herbaceous plant
Eight‐ to ten‐year old French hybrid Vidal 256 grapevines in southern Maryland produced berries about one‐third normal size but did not express any obvious leaf symptoms. Electron microscopy of negatively stained tissue‐dip preparations and sectioned material from such vines showed individual and membrane‐associated 28 nm spherical virus‐like particles and closteroviruslike particles. The spherical particles were characterized as an isolate of tomato ringspot virus (TomRSV‐G) that infected a wide range of herbaceous hosts by mechanical inoculation, but did not infect tomato, bean or petunia plants susceptible to the type strain of TomRSV. The closterovirus‐like particles did not react, by immunosorbent electron microscopy, with antisera to grapevine virus A (grapevine stem‐pitting associated virus of C onti et al. 1980) or the 2200 nm Swiss grapevine leafroll closterovirus (G ugleri et al. 1984).