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Comparison of Three Plant Rhabdovirus Isolates by Two Different Serological Techniques
Author(s) -
Adam G.,
Chagas C. M.,
Lesemann D.E.
Publication year - 1987
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1987.tb04412.x
Subject(s) - biology , antiserum , serology , virus , heterologous , virology , western blot , microbiology and biotechnology , antibody , biochemistry , genetics , gene
Three different rhabdovirus isolates, eggplant mottled dwarf virus (EMDV), tomato veinyellowing virus (TVYV) and a hitherto undefined isolate from tomato in Portugal (Tom‐P) were compared by two different serological techniques, immunosorbent electron microscopy (ISEM) and electro‐blot‐immunoassay (EBIA). Antisera were prepared either against purified virus or against partially purified virus preparations extracted by a one‐step procedure. Even the partially purified virus preparations yielded antisera that allowed unequivocal identification of two of the five structural virus proteins, G and N, in EBIA and were suitable for specific trapping of complete virus particles on electron microscope grids. With both serological techniques identical results were obtained indicating a close serological relationship between the three isolates tested. Cross‐reactions between the G proteins could be deduced from heterologous trapping of complete virus particles in ISEM. The cross‐reactions for the G proteins were substantiated by the EBIA‐results which indicated in addition cross‐reactions between the remaining three major structural virus proteins, N, M 1 , and M 2 , but also revealed significant differences in the molecular weights of the G and M 1 proteins between TVYV and the other two isolates. The results obtained indicate that Tom‐P is serologically closely related and in respect of protein molecular weights identical to EMDV, and that TVYV is not a separate virus but rather a strain of EMDV.