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Detection of the Grapevine Fanleaf Viruses Away from the Period of Vegetation
Author(s) -
Walter B.,
Etienne L.
Publication year - 1987
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1987.tb00499.x
Subject(s) - biology , cutting , horticulture , virus , vegetation (pathology) , botany , virology , medicine , pathology
Best conditions for the detection of AMV (Arabis Mosaic Virus) and GFV (Grapevine Fanleaf Virus) in grapevine tissues at different seasons in the year have been determined. Leaves, rootlets and wood shavings are good virus sources for ELISA. The commonly used nicotine solution can be replaced by a PBS‐ or Tris‐HCl buffer, as extraction medium, provided its molarity is high and it contains polyvinylpyrrolidone. In our conditions, detection of AMV and GFV was possible in leaves from May to October, There was no clear‐cut advantage in using extracts prepared either from leaves obtained from the top, middle or bottom parts of a plant. Away from the period of vegetation, the use of wood shavings allowed virus detection, even when cuttings were harvested a few months ago and kept at 6°C.