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The Development of an In Vitro Host‐Pathogen System Consisting of Maize Seedling Segments Infected with Ustilago maydis
Author(s) -
Hausen G.,
Beiderbeck R.
Publication year - 1987
Publication title -
journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.53
H-Index - 60
eISSN - 1439-0434
pISSN - 0931-1785
DOI - 10.1111/j.1439-0434.1987.tb00437.x
Subject(s) - ustilago , biology , seedling , benomyl , inoculation , botany , smut , gall , host (biology) , pathogen , horticulture , microbiology and biotechnology , fungicide , ecology , genetics , gene
The nodal segments of 7‐day‐old seedlings of maize were inoculated with sporidia of Ustilago maydis. At different times after inoculation (day 0 to day 9), the nodal segments were explanted to tissue culture media containing Benomyl. On one of the media, segments explanted at day 1 or later responded with the formation of galls carrying roots. In such galls Ustilago could complete its life cycle and form teliospores. Explantation at day 0 never led to gall or teliospore formation. Thefindings are discussed with respect to the possible presence of mycoboethins.